Gene transcriptions/Boxes/GC/Quiz

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The GC box is a lecture and an article as part of the gene transcription series and gene transcription course.

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Quiz

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1. Which of the following are phenomena associated with CpG sites?

cytosine nucleotide occurs next to a guanine nucleotide
"—C—phosphate—G—"
CG base-pairing
C and G are found on the same strand of DNA or RNA and are connected by a phosphodiester bond
cytosine being 3 prime to the guanine base
CpG islands
three hydrogen bonds

2. True or False, A repeating sequence of nucleotides that forms a transcription or a regulatory signal is called a box.

TRUE
FALSE

3. Several promoter elements have been previously identified in eukaryotes, but those cannot account for transcription from most RNA polymerase II-transcribed

4. True or False, The GC box is located in the distal promoter.

TRUE
FALSE

5. Which of the following is not a transcriptional characteristic of the GC box?

located between nucleotides -8 and +2 relative to the transcriptional start site (+1)
G/A/T-G/C+1-G-T/C-G-G-G/A-A-G/C
in eukaryotes
TATA-less genes
a consensus sequence

6. True or False, An AGC box contains the nucleotide sequence CGCC.

TRUE
FALSE

7. Which of the following are phenomena associated with the GC box?

transcription of A1BG
directs activator-dependent but TFIID-independent transcription
TATA box-less promoters
transcription start site
mediator dependence
core promoter
RNA polymerase II holoenzyme

8. True or False, The GC box is found in the promoter regions of about 1% of human genes.

TRUE
FALSE

9. Which of the following are phenomena associated with an Alu element?

a short stretch of 2-8 nucleotides
Arthrobacter luteus
TATA box-less promoters
occurs in large numbers in primate genomes
mediator dependence
short interspersed elements
there are over one million Alu elements interspersed throughout the human genome

10. True or False, A proximal promoter can be found within any core promoter.

TRUE
FALSE

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Research

Hypothesis:

  1. Various types of repeating boxes containing cytosine and guanine exist.

Control groups

This is an image of a Lewis rat. Credit: Charles River Laboratories.

The findings demonstrate a statistically systematic change from the status quo or the control group.

“In the design of experiments, treatments [or special properties or characteristics] are applied to [or observed in] experimental units in the treatment group(s).[1] In comparative experiments, members of the complementary group, the control group, receive either no treatment or a standard treatment.[2]"[3]

Proof of concept

Def. a “short and/or incomplete realization of a certain method or idea to demonstrate its feasibility"[4] is called a proof of concept.

Def. evidence that demonstrates that a concept is possible is called proof of concept.

The proof-of-concept structure consists of

  1. background,
  2. procedures,
  3. findings, and
  4. interpretation.[5]

See also

References

  1. ↑ Klaus Hinkelmann, Oscar Kempthorne (2008). Design and Analysis of Experiments, Volume I: Introduction to Experimental Design (2nd ed.). Wiley. ISBN 978-0-471-72756-9. http://books.google.com/?id=T3wWj2kVYZgC&printsec=frontcover.
  2. ↑ R. A. Bailey (2008). Design of comparative experiments. Cambridge University Press. ISBN 978-0-521-68357-9. http://www.cambridge.org/uk/catalogue/catalogue.asp?isbn=9780521683579.
  3. ↑ "Treatment and control groups, In: Wikipedia". San Francisco, California: Wikimedia Foundation, Inc. May 18, 2012. Retrieved 2012-05-31.
  4. ↑ "proof of concept, In: Wiktionary". San Francisco, California: Wikimedia Foundation, Inc. November 10, 2012. Retrieved 2013-01-13.
  5. ↑ Ginger Lehrman and Ian B Hogue, Sarah Palmer, Cheryl Jennings, Celsa A Spina, Ann Wiegand, Alan L Landay, Robert W Coombs, Douglas D Richman, John W Mellors, John M Coffin, Ronald J Bosch, David M Margolis (August 13, 2005). "Depletion of latent HIV-1 infection in vivo: a proof-of-concept study". Lancet 366 (9485): 549-55. doi:10.1016/S0140-6736(05)67098-5. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1894952/. Retrieved 2012-05-09.

External links

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Development status: this resource is experimental in nature.
Educational level: this is a research resource.
Resource type: this resource is a quiz.
Subject classification: this is a genetics resource.
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